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来源：爱够旅游网

Luciferase activities were standardized against renilla luciferase activity from a cotransfected control reporter (pRL-CMV–renilla luciferase) and were expressed as fold increases of the activity of reporter alone.

荧光素酶活性以共转染对照报告基因的海肾荧光素酶为标准化,测得荧光素酶的表达量相比单独只有报告子的活性成倍增加. Here we present evidence that GATA-2 has the capacity to physically associate with PLZF

Luciferase reporter assays were conducted as

described with pRL–CMV–renilla luciferase plasmids (Promega) monitoring transfection efficiencies.

荧光素酶检测系统是用pRL–CMV海肾荧光素酶载体来监测其转染率

Relative luciferase activities reflect duplicate values from a representation of no fewer than 2 independent experiments

相关荧光素酶活性展示了双份数据,它是从不少于两组实验中的代表中获得.

expression plasmids for Flag–GATA-2 and PLZF-RAR

Oligonucleotides were recovered by incubating with streptavidin–agarose beads。

solid bar，open bar

Expression plasmids for GATA-2 ，PLZF were used as indicated, in combination with GATA-1/Luc or mutant GATA-1/Luc reporter plasmid.

正如文中所说, GATA-2,PLZF的表达质粒用于与GATA-1/Luc或突变的GATA-1/Luc报告基因质粒结合.

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