专利名称：Analysis and separation of platelet-derived
growth factor proteins
发明人：Kunitani, Michael,Tran, An,Parker, Hugh申请号：EP08006174.0申请日：19971212公开号：EP1935901A1公开日：20080625
摘要：Methods for improving purification and quantification of platelet-derivedgrowth factor (PDGF) proteins having structural heterogeneity are provided. Preparationof substantially pure isoforms of these proteins is achieved using TSK sulfopropyl cationexchange chromatography and reverse phase high performance liquid chromatography.A reverse charged capillary zone electrophoresis method enables quantification ofsubstantially pure isoforms of these proteins resulting from endoproteolytic post-translational modifications. Compositions of the invention are substantially purifiedisoforms of secreted PDGF proteins having structural heterogeneity, more particularlypurified intact, single-clipped, and double-clipped isoforms of recombinant PDGF-BB.Pharmaceutical compositions comprising at least one of these substantially purifiedrecombinant PDGF isoforms and methods for their use in promoting wound healing arealso provided.
申请人：Novartis Vaccines and Diagnostics, Inc.
地址：4560 Horton Street Emeryville, CA 94608 US
国籍：US
代理机构：Bentham, Andrew
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